Abstract
The effects of ascorbic acid and L-Cysteine Hydrochloride (Cys-HCl) on growth of <em>Bifidobacterium bifidum</em> BB01 and BB03 were studied by using MRS broth as the control, the concentrations of ascorbic acid and Cys-HCl were both of 0.4, 0.6, 0.8, 1.0 and 1.2g/L. The result showed as follows: ascorbic acid 0.8g/L or Cys-HCl 0.6g/L on growth of <em>B. bifidum</em> BB01 and ascorbic acid 0.4g/L and Cys-HCl 0.4g/L on growth of <em>B. bifidum</em> BB03 had significant influence (p<0.05), respectively. The OD<sub>600</sub> of <em>B. bifidum</em> BB01 and <em>B. bifidum</em> BB03 reached 1.32, 1.32, 1.347 and 1.296 at optimal concentration of ascorbic acid and Cys-HCl mentioned above, while the control only reached 1.121 and 1.213 respectively.
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