Abstract
Glutamine synthetase (EC 6.3.1.2) catalyses the formation of glutamine from glutamate and ammonia coupled with the breakdown of ATP to ADP and phosphate. In the presence of ATP, glutamate and Mg, but in the absence of ammonia the enzyme forms a complex containing glutamate, ADP and phosphate; with ammonia, this complex forms glutamine, ADP, phosphate and free enzyme. The presence of ATP is necessary for the enzyme to bind glutamate, and for the reverse reaction ADP is required for the binding glutamine (7):
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