Effect of Aqueous Turmeric Extract on Lipid Peroxidation and Activity of Antioxidant Enzymes in Liver Tissue of Chick Embryo Treated with silver nanoParticles

Abstract

Turmeric extract has antioxidant, antibacterial, antifungal, antiviral, and anti-inflammatory effects, and in addition, curcumin present in turmeric extract can be a powerful protective factor against biochemical changes and cellular oxidative damages. In order to investigate the effect of turmeric aqueous extract on lipid peroxidation and the activity of antioxidant enzymes in the liver tissue of chick embryos treated with silver nanoparticles, 45 race Ross 308 fertilized eggs were randomly divided into control groups (single injection of 0.5 ml saline solution) and 4 treated groups. On the 10th day of incubation, treated groups of 1, 2, 3 and 4 received silver nanoparticles (a single injection of 0.5 ml silver nanoparticles, 200 ppm and 60 nm). On the 12th day of incubation, treated groups of 2, 3 and 4 received turmeric aqueous extract with concentrations of 100, 200 and 300 μg/ml (single injection of 0.5 ml turmeric aqueous extract). The injection was done in the amnion sac embryos. On the 20th day of incubation, the concentration of malondialdehyde and the activity of superoxide dismutase, glutathione peroxidase and catalase enzymes in liver tissue were measured by ELISA method. Statistical analysis was carried out by using one-way ANOVA and post-hoc Tukey tests (p<0.05). Compared to treated group 1, the tissue concentration of superoxide dismutase, glutathione peroxidase and catalase in treated groups 2, 3 and 4 significantly increased and the tissue concentration of malondialdehyde decreased significantly (p<0.05). Dose-dependent injection of turmeric aqueous extract dreduces the toxicity caused by silver nanoparticle and reduces oxidative stress and lipid peroxidation in liver tissue of chick embryos.