Effect of aqueous leaf extract of Macaranga barterion selected haematological and biochemical indices of carrageenan -induced paw edema in Wistar albino rats

Abstract

This research was designed to determine the anti- inflammatory and quantitative characterization of phenolic compounds in aqueous leaf extract of Macaranga barteri on carrageenan induced paw edema in wistar rats. Phenolic compound was characterize using GC-MS. Twenty wistar rats of both sexes weighing between 120-140g were grouped into 5 with four (4) rats per group. Group 1 served as the normal control which received distilled water, group 2 (negative control) rats received 1% carrageenan only, groups 3 and 4 were treated with aqueous leaf extract of M. barteri, group 5 was treated with diclofenac, 30 minutes after receiving 1% carrageenan, the changes in paw sizes were observed hourly for 3 hours. Rats were sacrificed and blood samples collected for haematological and biochemical analyses. Phenolic compounds detected were 2-nitro phenol (3.29 ppm) and 4-chloro-3-methyl-phenol (5.09ppm). Significant reduction in rat paws oedema was observed when rats in group 4 were compared with group 2 at 3hours with percentage oedema inhibition of 46.91. Mean ESR of rats in groups 2, 3 and 5 significantly increased and group 4 none significantly reduced when compared to control. Non-significant differences in mean WBC, neutrophil and lymphocyte counts, significant reduction of mean eosinophil counts in groups 2 and 3 and non-significant increase in group 4 when compared to control. Significant increase in mean CRP concentration was observed when group 2 was compared to control group and a significant reduction in group 4 treated with 500mg/kgb.wt. When compared to group 2. Mean concentrations of Il-6 and prostaglandin significantly increased in all the groups when compared to control. Aqueous leaf extract of M. barteri demonstrated near average percentage paw oedema inhibition and mild anti-inflammatory potential at 500mg/kg b.wt.