Effect of aqueous extract of shallot (Allium ascalonicum) on inhibition of growth of Pseudomonas aeroginosa

Abstract

Shallot from Liliacea are the oldest plants that are used for treatment of different disease such as bacterial, parasitic, fungal infections and also viruses. In addition, Pseudomonas aeroginosa is the most important human pathogen with invasive nature that causes different infections especially in cases with abnormal defence. It is resistant to many antibiotics, makes some problems in treatment. This study was done to evaluate the effect of shallot on pseudomonas aeroginosa. The undergoround bulbs of this plant were percolated in distilled water for 7 days, heat-dried (50°C in a dry oven) and the crystalline products were isolated. 10, 20, 30, 40 and 50% w/v of aqueous solutions of these crystal were prepared. Microorganism used in this experiment was pseudomonas aeroginosa (PTCC1074). The above suspension was cultured homogenously in Muller Hinton Agar, and the antibacterial property of extracts was then investigated by well assay method. The zone of growth around each well was assessed after 24-48 hours. The results of this study indicated that pseudomonas aeroginosa (PTCC1074) was sensitive to this extract, total results showed that antimicrobial effects was from bacteriocide type and the most effect was in aqueous solution about 50%. In addition, the inhibitory growth zone diameter was in 30 mm to 50 mm, so that not only we can use this extract for its anti-microbial effect on pseudomonas aerogenes as people use it in their food but also it can be a wonderful study in pharmacology field for scientists.