Effect of aqueous extract of Rosemary officinalis on cytotoxicity of CCL4 induced albino male mice

Ruqaya M Ibrahim

doi:10.24126/jobrc.2018.12.1.557

Abstract

This study focused the line on the effect of aqueous extract of Rosemary officinalis, as well as, effect of toxic compound CCL4, on micronucleus formation and mitotic index assay in albino male mice. This work started at September 2017 at Biotechnology Research center \Al-Nahrain University, by using 20 albino male mice. The result indicated that aqueous extract of rosemary caused significant increased in mitotic index and decrease micronucleus formation for two doses tested 50,100 mg/kg in comparison with negative and positive controls, also the results revealed that CCL4 showed significant mutagenic action on biological system of treated mice by increased frequency of micronucleus formation and decreased the percentage of mitotic index in bone marrow cells. Pre-and post –treatment between aqueous extract and CCL4 were also made. The results of pre and post treatment with rosemary extract were also caused a significant decreased in micronucleus formation and increase the percentage of mitotic index for two doses 50,100 mg/kg in comparison with its corresponding controls which caused increased in the frequencies of micronucleus formation and decrease the percentage of mitotic index in bone marrow cells. Conclusions: Rosemary officinalis enhanced immunity, reduced mutagenic effects against cytotoxicity of CCL4.

Highlights

Journal of Biotechnology Research Centerٌٕ‫ذ انكبسب‬ٚ‫ كهٕس‬ٙ‫ت نًشكب سببػ‬ٕٚ‫ت انخه‬ًٛ‫م انجبم ػهٗ انس‬ٛ‫ لأساق اكه‬ٙ‫ش انًسخخهص انًبئ‬ٛ‫حبث‬ ‫ط‬ٛ‫ ركٕس انفئشاٌ انب‬ٙ‫ ف‬CCL4
‫شة‬ٛ‫ انُٕٖ انصغ‬،ٕ٘‫ الاَمسبو انخه‬،ٌٕ‫ذ انكبسب‬ٚ‫ كهٕس‬ٙ‫ سببػ‬CCL4 ،‫م انجبم‬ٛ‫ إكه‬:‫انكهًبث انذانت‬ Introduction The relationship of humans and animals with plants obviously originated with the beginning of life on earth [1] when these plants abounding much of the shelter, oxygen, food and medicine needed by higher life forms [2]
Rosemary (Rosmarinus officinalis L.), which belongs to the family Lamiaceae, is an aromatic, evergreen, high shrub with upright stems, whitish -blue flowers and dark green leaves [8].The therapeutic nature of rosemary extract is related to its content of important active constituents mainly diterpenes; phenolic acids and flavonoids that derived from two frequent flavones: apigenin and luteolin [9]

Summary

Journal of Biotechnology Research Center

ٌٕ‫ذ انكبسب‬ٚ‫ كهٕس‬ٙ‫ت نًشكب سببػ‬ٕٚ‫ت انخه‬ًٛ‫م انجبم ػهٗ انس‬ٛ‫ لأساق اكه‬ٙ‫ش انًسخخهص انًبئ‬ٛ‫حبث‬ ‫ط‬ٛ‫ ركٕس انفئشاٌ انب‬ٙ‫ ف‬CCL4. Remedial plants have long played essential roles in the treatment of diseases all over the world [3] Some of these properties are antimicrobial, anti-cancer, anti-diabetic [4], anti-atherosclerosis [5], immunomodulatory [6], in addition to use as source for a extensive variety of natural antioxidants [7]. Prolonged exposure to carbon tetrachloride induced histopathological features such as inflammatory leucocytes’ infiltration, necrosis , fibrosis, cirrhosis and sometimes may lead to tumors [18]. It induced liver fibrosis, enhanced lipid peroxidation, increases alanine aminotransferase (ALT) and causes collagen deposition in liver tissue [19]. Poisoning by CCl4 induced toxic injury to both liver and kidney [20]

Materials and Methods

Findings

Rosemary aqueous extract