Effect of antibiotic type and vegetable species on antibiotic accumulation in soil-vegetable system, soil microbiota, and resistance genes

Abstract

Antibiotic accumulation in soil and plants is an escalating problem in agriculture and is receiving increasing attention. However, the effect of plant species on the fate of different types of antibiotics in a soil-vegetable system and soil resistome has not been adequately explored. To this end, greenhouse pot experiments were conducted to simulate contamination by ciprofloxacin (CIP), oxytetracycline (OTC), sulfamethoxazole (SMZ), and tylosin (TY) at 1 mg kg−1 in the soils in which cabbage, endive, and spinach were grown. We investigated antibiotic persistence in soils and accumulation in vegetables (i.e., spinach, endive, and cabbage), microbial community profiles, and the abundance of 17 antibiotic resistance genes (ARGs) in contaminated soils. After 40 days, the residues of CIP and OTC in soil and their accumulation in vegetables were significantly higher than those of SMZ and TY. Of all vegetables, spinach had the highest antibiotic accumulation. Further, antibiotic contamination had no significant effect on soil microbial abundance; however, soil microbial diversity significantly decreased in soils amended with TY. The antibiotic type more significantly affected microbial composition than the kind of vegetable species. The relative abundances of some ARGs significantly increased in contaminated soils. Particularly, in endive soil, quinolone-associated cmlA, cmlA2, and qnrS1 increased with CIP contamination, OTC contamination increased tetG2 and otrA, SMZ increased sul1, and TY increased macrolide-related carB and msrc-01 relative abundance. However, some individual ARGs declined upon antibiotic contamination. Our results indicated that antibiotic type and vegetable species jointly shape the profiles of soil microorganisms and ARGs.