Abstract
To explore whether the antarctic krill oil has an impact on astaxanthin ester absorption process, provide scientific basis and theoretical support for the development and application of Antarctic krill oil in functional food or special medical food. Methods: 150 Wistar male rats were randomly divided into 5 groups: blank control group, 0.208g oil/kg.bw Antarctic krill oil group, 1.25mg/kg body wt of astaxanthin group, 1.25mg/kg body wt of astaxanthin+0.208g oil/kg.bw Antarctic krill oil group and 1.25mg/kg body wt of astaxanthin+0.208g oil/kg.bw Fish oil group.Samples were collected at ten time points(0, 0.5,1,2,4,8,10,12,16,24h) after administering a single oral dose corresponding to rats via the intragastric gavage mode and the astaxanthin concentrations in samples were detected using Hplc.Results: The maximum concentration of astaxanthin in the serum of ASTA- krill oil group was delayed by 6 h compared with the ASTA group. The highest concentration of ASTA- krill oil group in serum was was 5.3 times of ASTA- Fish oil group, which was 8.4 times that of ASTA group. The serum AUC values of ASTA-krill oil group,the ASTA group and the ASTA-Fish oil group were 108.39±2.34ug∙h/L, 14.35±0.21 ug∙h/L, 23.39±0.46 ug∙h/L, respectively., reflecting that the bioavailability of ASTA- krill oil in rats is higher than that of ASTA group, which is better than ASTA- Fish oil .Conclusion: The serum absorption rate of the ASTA- krill oil group are excellent. Antarctic krill oil promotes the bioavailability of astaxanthin and provides scientific basis and theoretical support for the development and application of functional foods or special medical foods.
Highlights
Antarctic krill (Euphausia superba), belonging to the arthropods (Arthropoda), Crustacea, Euphausiacea, Euphausiidae, Euphausia, living in a cluster The single species of plankton in the Southern Ocean has a huge biomass of about 6.5 to 1 billiontons
The corresponding gradient elution procedure are shown in Table 1.The total chromatography time was 35.0 min and retention times for astaxanthin was 10.40 min(Fig.1)
Linearity of the calibration curve, lower limit of quantification (LLOQ), and limit of detection (LOD) Standard curves were established by plotting of chromatogram peak areas of astaxanthin
Summary
Antarctic krill (Euphausia superba), belonging to the arthropods (Arthropoda), Crustacea, Euphausiacea, Euphausiidae, Euphausia, living in a cluster The single species of plankton in the Southern Ocean has a huge biomass of about 6.5 to 1 billiontons. As a new treasure trove of marine quality protein and active lipids, Antarctic krill has become the most important development target of China's oceanic strategic resources. Ultraviolet spectrophotometry, high performance liquid chromatography and thin layer chromatography are used to quantitatively analyze astaxanthin and astaxanthin esters, among which high performance liquid chromatography is used. This experiment used astaxanthin ester extracted from Haematococcus pluvialis as raw material, and compared with Antarctic krill oil and astaxanthin ester by gavage in rats, systematically investigated the intake of Antarctic krill. Astaxanthin is a natural antioxidant, and its antioxidant capacity is much higher than other active substances such as
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