Abstract
Objective To investigate the hemorheology changes in flap ischemia reperfusion injury and the protective effect of angelica polysaccharides (APS).Methods Fifty adult SD rats were divided into a sham group,a control group,a 2 mg/kg APS group,a 4 mg/kg APS group,and a 8 mg/kg APS group.After a 5 cm × 4 cm epigastric flap was elevated,flaps in the sham group were sutured back into its place without inducing any ischemia.Flaps in the control group and APS treatment groups were subject to a 6-hour ischemic period.Five minutes prior to the repeffusion,0.2 ml saline was injected via intraperitoneal route in the control group while 0.2 ml of corresponding doses of angelica polysaccharides were injected in the APS groups.Following a 12-hour reperfusion,the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the flap tissue were measured.Polymorphonuclear neutrophils (PMN) in the peripheral blood were counted.Flow cytometry was used to detect the expression of CD11a/CD18 and CD11b/CD18 on the polynorphonuclear neutrophils.Hemorheology parameters were detected in the rats of each group.Results Compared with the control group,8 mg/kg APS group showed significant decrease of MDA content and CD11a/CD18,CD11b/CD18 expression levels after 12-hour repeffusion (P ＜ 0.05).The level of SOD increased significantly (P ＜ 0.05).The PMN number,the low shear viscosity,the middle shear viscosity and high shear viscosity of the APS groups were significantly lower than those of the control group (P ＜ 0.05).Plasma viscosity,RBC aggregation index of the APS groups were not significantly different from the control group (P ＞ 0.05).Conclusion APS can reduce ischemia reperfusion injury of skin flaps in rats via reducing the number of neutrophils,down-regulating the expression of CD11a/CD18 and CD11b/CD18 on neutrophil surface,and decreasing blood viscosity,so as to improve blood supply of the flap. Key words: Angelica polysaccharides; Flaps; Ischemia reperfusion injury; Hemorheology
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