Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models.

Emilia Piosik,Tomasz Martyński,Dorota Chełminiak-Dudkiewicz,Marta Ziegler-Borowska

doi:10.3390/ijms22052467

Abstract

An adsorption process of magnetite nanoparticles functionalized with aminated chitosan (Fe3O4-AChit) showing application potential in nanomedicine into cell membrane models was studied. The cell membrane models were formed using a Langmuir technique from three selected phospholipids with different polar head-groups as well as length and carbon saturation of alkyl chains. The research presented in this work reveals the existence of membrane model composition-dependent regulation of phospholipid-nanoparticle interactions. The influence of the positively charged Fe3O4-AChit nanoparticles on a Langmuir film stability, phase state, and textures is much greater in the case of these formed by negatively charged 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DPPG) than those created by zwitterionic 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC). The adsorption kinetics recorded during penetration experiments show that this effect is caused by the strongest adsorption of the investigated nanoparticles into the DPPG monolayer driven very likely by the electrostatic attraction. The differences in the adsorption strength of the Fe3O4-AChit nanoparticles into the Langmuir films formed by the phosphatidylcholines were also observed. The nanoparticles adsorbed more easily into more loosely packed POPC monolayer.

Highlights

The adsorption process of the aminated chitosan-coated magnetite nanoparticles (Fe3O4-AChit nanoparticles) with application potential in nanomedicine into the phospholipid Langmuir monolayers made of DPPG, DSPC, and POPC constituting the cell membrane models was investigated
The π-A isotherms and Brewster angle microscope (BAM) images showed that the influence of the Fe3O4-AChit nanoparticles on the stability, phase behavior, and textures of the phospholipid Langmuir films was much greater in the case of those formed by DPPG than those created by DPPC, DSPC, and POPC
The differences in an interaction strength of the Fe3O4-AChit nanoparticles with the monolayers made of DPPG representing the phosphatidylglycerols and those formed by the phosphatidylcholines are caused presumably by the stronger electrostatic attraction of the positively charged nanoparticles to the negatively charged DPPG molecules than to the zwitterionic DPPC, DSPC, or POPC molecules

Summary

Introduction

Nanomaterials such as graphene, silicene, germanene, nanotubes, and nanoparticles thanks to their novel intrinsic properties have attracted the attention of researchers from both academic and industrial sectors in the chemical, environmental, optoelectronic, and medical fields [1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17]. The presence of the Fe3O4-AChit nanoparticles in the water subphase does not cause a significant shift of the DSPC compression isotherms in the direction of the larger mean molecular areas like it takes place in the case of DPPG (Figure 3a). The change of their shape is observed at the initial stage of the surface pressure increase (Figure 3a, inset). This can enhance the zwitterionic phospholipid-positively charged aminated chitosan interaction

Materials and Methods

Phospholipids

Brewster Angle Microscopy Observations

Penetration Experiments

Findings

Conclusions