Abstract
Using a probe and procedure developed for the Abbott TDx analyzer, we investigated the influence of albumin on fluorescence polarization of amniotic fluid. Polarization readings increased when albumin was added to either "mature" or "immature" amniotic fluids. Albumin added to a fraction rich in lamellar bodies (10,000 X g pellet) greatly increased polarization readings, but albumin added to the lamellar-body-free fraction resulted in only small increases. Adding increasing amounts of the lamellar-body-rich fraction from mature and immature pools to a constant amount of albumin decreased the polarization readings. We saw no correlation between amniotic fluid polarization and albumin concentration but found a significant correlation between polarization values and either total extractable phospholipids (r = 0.725, P less than 0.001) or the ratio albumin/total extractable phospholipids (r = 0.784, P less than 0.001). The concentration of albumin in amniotic fluid was variable and correlated poorly with gestational age.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call