Abstract
The effects of aging on intestinal absorption of zinc and L-histidine were investigated in adult (10-month-old) and senescent (30-month-old) Wistar rats' brush-border membrane vesicles isolated from jejunum and ileum. Kinetic parameters of the zinc transport by the jejunal brush-border membrane were J max=126 ± 24 nmol·min −1·mg −1 protein and K m=490 ± 126 μM (10-month-old rats, n=7). The transport of zinc was the same in the jejunum and the ileum of adult animals. In senescent rats, the zinc uptake was significantly lower in the distal part of the intestine than in the proximal one. A comparison of zinc uptake in 10- and 30-month-old rats showed that the transport capacity of the jejunum did not change with age but the ileal transport capacity decreased by 50%. This reduced uptake was associated with an increased cholesterol content of the brush-border membrane. The major site of L-histidine absorption was the jejunum, in both the 10- and 30-month-old animals. L-Histidine was co-transported with Na +. The kinetic parameters of the L-histidine carrier in the presence of Na + were J max = 6.5 ± 1.0 nmol·min −1·mg −1 protein and K m=190 ± 29 μM in the jejunum of 10-month-old rats ( n = 12). Increasing the extra-vesicular concentration of zinc (0 → 1 mM) reduced the uptake of L-histidine, and conversely increasing the concentration of L-histidine (0 → 1 mM) reduced that of zinc: these was no evidence of transport of a complexed form [zinc-L-histidine] in brush-border membranes of the small intestine. During aging, the transport capacity of L-histidine by the jejunum decreased, whereas the ileal transport capacity was conserved. The modifications of absorptive capacity for zinc and L-histidine at the membrane level (loss of ileal function for zinc, and loss of jejunal function for amino acid) indicate that the normal aging of intestinal epithelial cells cannot be regarded as a decline in the overall transport of nutriments but as a combination of highly specific modifications of the various transport systems.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
