Abstract
The incorporation of ( 3H) orotic acid into RNA by hepatocytes from rats of various ages was studied. A 37% decrease in the incorporation of ( 3H) orotic acid into RNA was observed by hepatocytes isolated from 30-month-old rats compared to 12-month-old rats. There was no age-related difference in the incorporation of ( 3H) orotic acid into acid-insoluble material by hepatocytes. The incorporation of ( 3H) orotic acid into UTP by hepatocytes was determined by high pressure liquid chromatography. The ( 3H) orotic acid was rapidly converted to UTP by hepatocytes, and the specific activity of the UTP reached a steady state after 10 min of incubation. There was no significant difference in the specific activity of the UTP in hepatocytes isolated from rats ranging in age from 6 to 25 months. Therefore, the age-related decrease in the incorporation of ( 3H) orotic acid into RNA by hepatocytes is not due to an age-related difference in the uptake or conversion of ( 3H) orotic acid into UTP.
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