Effect of Aerobic Training and Use of Nanomicelle Curcumin on Gene Expression of GPX, Cat, SOD and MDA Enzymes in Heart Tissue of Balb C Female Mice with Induced Breast Cancer in Treatment Phase with Doxorubicin

Abstract

Introduction:Doxorubicin is a universal drug in treatment of all types of cancers, which is limited in its use due to various side effects in body tissues, so the present study investigated effect of aerobic exercise and use of curcumin nanomicelles on gene expression of the enzymes (GlutathionePeroxidase) GPX, (Catalase) Cat (Superoxidedismutase) SOD and (Malondialdehyde) MDA was investigated in the heart tissue of BalbC female rats with induced breast cancer in the treatment phase of doxorubicin.
 method: This research was practical, it was experimental in terms of control of variables and it was a laboratory in terms of place of implementation. Thirty-six BalbC mice were randomly divided into 6 groups (n=6): healthy, patient, doxorubicin patient, doxorubicin patient and aerobic exercise, doxorubicin patient and nanocurcumin supplement, and doxorubicin patient, aerobic exercise and supplementation. Breast cancer induction was done with MC4-L2 cells. Doxorubicin 2mg/kg was injected subcutaneously 6 times on days 1,7,14,21,28 and 35. Aerobic program consisted of 5 sessions per week for 42 days with an increase in the speed of the treadmill every two weeks, including 14, 16 and 18meters per minute and the use of nanocurcumin at a dose of 100mg/kg.The data collected with SPSS version16 and two-way analysis of variance and for the normality of the frequency distribution with skewness and non-parametric Kolmogorov-Smirnov test were performed with a significance level of P ≤ 0.05.
 Results: Six weeks of aerobic training had no effect on the expression of GPX, MDA and SOD genes in the target groups, but it significantly increased the expression of Catgene (P ≤ 0.05). The use of nanocurcurmin supplement for 6 weeks decreased the expression of MDA gene and significantly increased the expression of GPX, SOD and Catgenes. Six weeks of aerobic training along with nanocorcurmin supplementation significantly increased Cat gene expression (P≤0.05), but had no effect on GPX, MDA and SOD gene expression.
 Conclusion: Aerobic exercises and the use of nanocurcurmin supplement separately or at the same time can be effective in reducing the cardiac toxicity of doxorubicin in the patients with breast cancer.