Effect of additives on the behavioural properties of tannin acyl hydrolase

Abstract

The effect of metal ions on tannase activity was studied. One mM Mg +2 or Hg + activated tannase activity. Ba +2, Ca +2, Zn +2, Hg +2 and Ag + inhibited tannase activity at 1.0 mM concentration and Fe +3 and Co +2 completely inhibited tannase activity. Ag +, Ba +2, Zn +2 and Hg +2 competitively inhibited tannase activity. Among the anions studied 1 mM Br − or S 2O 3 −2 enhanced tannase activity. Tween 40 and Tween 80 enhanced tannase activity whereas Tween 60 inhibited tannase activity. Sodium lauryl sulfate and Triton X-100 inhibited tannase activity. Urea stimulated tannase activity at a concentration of 1.5 M. Among the chelators chosen for the present study, 1 mM EDTA or 1,10- o-phenanthrolein inhibited tannase activity Dimethyl sulphoxide and β-mercaptoethanol inhibited tannase activity at 1 mM concentration whereas soybean extract inhibited tannase activity at concentrations varying from 0.05 to 1.0% (w/v). Among the nitrogen sources selected ammonium ferrous sulfate, ammonium sulfate, ammonium nitrate and ammonium chloride enhanced tannase activity at 0.1% (w/v) concentration.