Effect of adding growth regulators benzyl adenine (BA) and kinetin (Kin) on production of potato (spunta) micro tuber

Abstract

This experiment was carried out in plant tissue culture laboratory a part of bio technology research center (BTRC) , while the main aim for this experiment was to investigate the effects of using plant growth regulators on potato micro tuber formation for Sponta potato variety throw plant tissue culture method , In the beginning micro propagation operations were done to the target potato variety in order to obtain enough number of plants samples in free contamination MS media , while different concentrations of BA and Kin plant growth regulators ( 0 , 1 , 2 , 3 mg/l ) were used in this experiment , the plant growth regulators BA ,Kin were added to MS media contain 60g/l sucrose during micro tuber formation stage . the results of this study showed that the treatment of (5mg/l Kin ) obtained the best results and this treatment gave the highest tuber production compared with other treatments , furthermore this treatment ( 5mg/l Kin) gave the highest number of produced potato tuber and the highest weight of produced tuber compared with other used treatments , while the average number of produced micro tubers arrived to 4.9 tuber for each plant and the average weight for obtained potato tuber arrived to 0.84 g which was higher than other weight of micro tuber produced from the other treatments .