Effect of Acrylamide on Ovaries of Female Albino Rats and Possible Protective Role of Vitamin E

Abstract

Abstract Background: Acrylamide (ACR) is a chemical substance with a very wide range of uses and it accounts for one of the major health concern and one of the most important contam-inants occurring in foods heated at high temperatures so human exposure to ACR is high. Vitamin E is the most important lipid-soluble vitamin presents in the nature and one of the basic vitamins in biological systems and plays a vital role in many physiological processes. Aim of Study: To investigate the Potential protective effects of Vitamin E on Acrylamide induced ovarian damage in female albino rats through histological examination by light microscope. Material and Methods: This study was conducted in Human Anatomy and Embryology Department in Sohag University during 2019 forty-five female virgin albino rats weighing 180-300g were used and randomly divided into three equal groups: Control group; ACR group was given 10 mg/kg body weight of acrylamide for by oral gavage for 3 weeks; the third group was given ACR at a dosage of 10 mg/kg/d, and vitamin E at a dose of 100mg/kg body weight for 3 weeks orally then sacrificed by cervical dislocation Ovaries were excised for light microscopic study and mor-phometric study. Results: Oral administration of ACR induced marked cystic ovarian changes, degenerative changes in zona pellucida, Granulosa cells and oocytes detected by light microscope this study reported that supplementation of a diet with Vit E provided antioxidant defense against the toxicity of ACR in ovary. Conclusions: These results concluded that acrylamide combined with vitamin E in comparison with acrylamide induced less ovarian damage and this protective effect might be due to the antioxidant activity of Vit E.