Effect of Acarbose and olive leaf extract (Oleuropein) on Glycemic index and Antioxidant Status in High Fructose and H2O2 Exposed Rats (Parts-II)

Abstract

This study was carried out to investigate the effect of acarbose or combination of acarbose and Oleuropein (OLEU) on glycemic index and antioxidant status of male rats exposed to high fructose(40%) and 0.05% hydrogen peroxide H2O2 Thirty-two adult male rats were randomly divided into four equal groups: control group (CC); T1( HFr-H2O2) group were exposed to high fructose (40%) in drinking water and given orally 0.05% H2O2; T2: 30mg/kg of acarbose were given orally to HF-H2O2 treated rats, while combination of acarbose and Oleuropein (50mg/kg. B.W) were given orally to (HFr-H2O2) treated rats in group T3. Blood samples were taken at zero time and after two months of the experiment for measuring some parameters related to glycemic index and antioxidant status. The results declared an elevation in the glycemic index (insulin and glucose concentrations and insulin resistance (IR)) and malondialdehyde (MDA) and depression in glutathione (GSH) concentrations in T1 group at the end of experiment comparing to other treated groups. Antioxidant status and glycemic index were restored after acarbose or combination of acarbose and Oleuropein treatment characterized by significant depression of serum glucose, insulin concentrations and IR, and alleviation of oxidative stress clarified by depression of serum MDA with elevation in serum GSH concentration in groups T2 and T3. On conclusion, the current study documented the oxidative and hyperglycemic effect of fructose and H2O2 that can be ameliorating by oral administration of acarbose. Besides, combination of OLUE supplement with acarbose possessed additive effects.