Abstract
The effect of a mixer in the gradient elution device on the variations in the concentration of eluents has been studied. The functions that characterize real concentration changes have been derived and compared with those describing programmed changes in concentration. On this basis the magnitudes of errors have been determined as a function of the mixer volume and the flow-rate. A method has been developed that permits one to correct the functions programming the operations of gradient elution devices containing mixers in such a manner as actually to obtain eluents of programmed composition. The correctness of the derived relationships has been verified experimentally.
Published Version
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