Effect of 6-Benzylaminopurine and Honey for in Vitro Shoot Initiation of Mangosteen Seed Explant From Riau, Indonesia

Abstract

Mangosteen (Garcinia mangostana L.) is a tropical plant that has export value because it has a rich of antioxidant xanthone that can be used for medicine as an anti-cancer agent. Mangosteen (Garcinia mangostana L.) from Riau, Indonesia has many superiorities such as grow in the marshes and tolerant to acid soil (peat soil). One of the alternative methods to produce large number and uniform mangosteen seedlings in a short period can be done using in vitro culture technique. The purpose of this research was to determine the optimal concentration of BAP and honey in shoot initiation of mangosteen explant from seed that had been divided into four parts and also to determine the best combination for initiation mangosteen shoots using in vitro method. This research used a Completely Randomized Design (CRD) with the concentration of BAP (3; 7 mg/l) and honey (3; 6; 9 ml/l) or in combination on MS medium with five replications. The result showed that the highest number of shoots (14.60) formed on MS medium supplement with 7 mg/l BAP + 3 ml/l honey. The combination between 3 mg/l BAP + 3 ml/l honey produced the tallest shoots (5.10 cm) and the highest number of leaves (3.20 leaves) on MS medium supplement with 3 mg/l BAP + 9 ml/l honey. In this study, we have successfully presented the effect of 6-benzyl amino purine and honey for in vitro shoots initiation from mangosteen seed explant (Garcinia mangostana L.) which taken from Riau Indonesia.