Abstract
An experiment on in vitro propagation of strawberry was carried out at Plant Breeding and Biotechnology Laboratory of Agrotechnology Discipline, Khulna University, Khulna, Bangladesh during the period of February to November, 2015. In the experiment, runners of two strawberry cultivars viz. BARI Strawberry-1 and Modern Strawberry-5 (Festival) were cultured on MS media supplemented with different concentrations of BAP (1.0, 1.5 and 2.0 mgL-1) and Kin (0.1, 0.5 and 1.0 mgL-1) for multiple shoot regeneration. The experiment was laid out in Completely Randomized Design (CRD) with three replications. The highest number of shoots (10.41) was obtained from Modern Strawberry-5 and maximum shoot length (13.62 mm) obtained from BARI Strawberry-1. Maximum number of shoots (13.33) and shoot length (24.29 mm) were recorded from MS media containing 1.0 mgL-1 BAP and 1.0 mgL-1 Kin. Then all the regenerated plantlets were cultured on MS media containing 2.0 mgL-1 IAA for root initiation. Greater reduction in vigor was observed in Modern Strawberry-5 (Festival) when they were transferred to root induction media. Ex vitro survivability of the complete plantlets varied from 36.67 to 6.67%. Maximum survivability of both varieties 36.67% (BARI Strawberry-1) and 26.67% (Festival) were recorded from MS media supplemented with 1.0 mgL-1 BAP and 1.0 mgL-1 Kin. For in vitro micropropagation, BARI Strawberry-1 may be better and MS media supplemented with 1.0 mgL-1 BAP and 1.0 mgL-1 Kin may be better.
Highlights
Strawberry (Fragaria ananassa Duch.) is a widely grown plant species of the genus Fragaria, belonging to the family Rosaceae
Collection of strawberry cultivars BARI Strawberry-1 was collected from Ankur nursery, Jessore and Modern Strawberry-5 (Festival) was collected from Modern Horticultural Center, Nator
Effect of variety on shoot multiplication and shoot length For in vitro shoot tip culture of strawberry, no significant difference was obtained for shoot regeneration between the at early stage (Table 1), but the multiplication rate of the shoots at the second sub-culture (60 Days after culture (DAC)) varied significantly for the varieties whereas they showed no significant variation at first and third sub-culture (30 DAC and 90 DAC)
Summary
Strawberry (Fragaria ananassa Duch.) is a widely grown plant species of the genus Fragaria, belonging to the family Rosaceae. Effect of variety on shoot multiplication and shoot length For in vitro shoot tip culture of strawberry, no significant difference was obtained for shoot regeneration between the at early stage (Table 1), but the multiplication rate of the shoots at the second sub-culture (60 DAC) varied significantly for the varieties whereas they showed no significant variation at first and third sub-culture (30 DAC and 90 DAC).
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