Effect of 2,4-d on embryogenic callus induction and evaluation of G418 on growth inhibition in rice calli

Abstract

An efficient in vitro callus induction study in rice genotypes Kitaake and PR124 was carried out using different concentrations of 2,4-D. Effect of 2,4-D was studied using four different concentrations in Kitaake (1, 2, 3, 4 mg/l) and in PR124 (1, 1.5, 2, 2.5 mg/l). This study found that Kitaake exhibited high frequency of callus induction on MS (Murashige and Skoog) medium supplemented with 2 mg/l 2,4-D, whereas in PR124, 1.5 mg/l concentration of 2,4-D was found to be the best for calli induction. Callus induction frequencies in Kitaake and PR124 were found to be 85 % and 64.4 %, respectively. Further, the study demonstrated that the growth of rice calli was not affected at lower concentrations of G418 and necrosis of calli occurred at 100 mg/l. These optimized concentrations of 2,4-D and G418 would be helpful to obtain desirable transformants in rice through Agrobacterium-mediated genetic transformation.