Abstract
The effects of α-linolenic acid (ALA) on the proliferation and adhesion of probiotics would be investigated in the present study. Effects of ALA on intestinal flora were studied by animal fecal anaerobic fermentation system in vitro, which were analyzed by high-throughput sequencing. Results showed that treatment with ALA could promote the proliferation of probiotics Lactobacillus and Bifidobacterium, and inhibit the growth of harmful bacteria Enterococcus and E. coli. ALA restored the abnormal intestinal flora caused by high-fat diet, which was beneficial to the improvement of intestinal flora structure. In addition, adhesive characteristics of probiotics to epithelial colon cells NCM460 were detected by plate counting and Gram staining, which indicated that ALA promoted adhesion of probiotics to colonic cells. In conclusion, ALA could promote the proliferation and adhesion of intestinal probiotics, which provides a basis for ALA to exert the healthy activities of intestinal probiotics.
Highlights
The effects of α-linolenic acid (ALA) on the proliferation and adhesion of probiotics would be investigated in the present study
Bifidobacterium bifidum and Lactobacillus acidophilus have been proved to be the important probiotics, and adhesion of probiotics is a necessary prerequisite for probiotics to play their physiological functions (Zhang, 2019; Bai et al, 2012; Kotzamanidis et al, 2010)
Treatment with ALA has a positive effect on the proliferation of probiotics
Summary
The effects of α-linolenic acid (ALA) on the proliferation and adhesion of probiotics would be investigated in the present study. Results showed that treatment with ALA could promote the proliferation of probiotics Lactobacillus and Bifidobacterium, and inhibit the growth of harmful bacteria Enterococcus and E. coli. Studies have shown that probiotics play a beneficial role in intestinal health by inhibiting growth of harmful bacteria and enhancing host intestinal mucosal barrier (Liu et al, 2016). Effects of ALA on intestinal micro-ecology of animals on high-fat-diet, and adhesion ability of probiotics has not yet been reported. Effects of ALA on intestinal flora of rats on high-fat-diet would be investigated in this study exploiting the in vitro animal fecal anaerobic culture system, as well as the effect of ALA on adhesion of probiotics with colonic epithelial cells NCM460. The present study will provide experimental evidence for candidates of ALA as a functional food ingredient to improve the intestinal health
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