EFFECT GROWTH REGULATORS AND STATE OF MEDIUM ON MICROPROPAGATION OF ADHATODA VASICA (NEES.)

Abstract

The experiment was conducted at Plant Biotechnology Laboratory of Khulna University, Khulna, Bangladesh to study the effect of auxin and cytokinin on shoot induction and subsequent rooting from shoot tip of Adhatoda vasica. For shoot proliferation, MS medium was supplemented with BA and Kinetin, ranging from 0.1-2.0 mgL-1 and for root induction, both solid and liquid MS media fortified with NAA or IBA 0.1-2.0 mgL-1 were employed. Maximum number of usable shoots was found on the medium containing 0.5 mgL-1 BA (1.86±0.52) and 1.5 mgL-l Kinetin (1.18±0.34). Root induction on solid medium showed that only one concentration of NAA (0.1 mgL-1) and 1.0, 1.5 and 2.0 mg/l of IBA responded in rooting, but the highest number of roots (6.22±1.79) with the highest average root length (0.88±0.25 cm) were observed on solid medium supplemented with IBA 1.0 mgL-1. All the concentrations of IBA and NAA responded in rooting on liquid medium with maximum number at the concentration of 1.5 mgL-1 NAA (16.79±5.3) and 1.0 mgL-1 IBA (13.1±3.78). The healthy roots were observed in liquid medium at the concentration of 0.1 mg/l NAA. In vitro regenerated plantlets were transferred to sterile soil containing 75% soil and 25% sand and then successfully established under natural condition.