Effect and Functional Mechanism of the Action of Exogenous Gibberellin on Flowering of Peach

Abstract

This study was conducted to assess the effect of gibberellin and its possible mechanism of action on peach flower formation. At flower induction, 100 mg L −1 of gibberellic acid 3 (GA 3) was sprayed on the leaves of peach [ Prunus persica (L.) Batsch.] cv. Bayuecui. Using anatomy, immunohistochemistry, and semi-quantitation, the in situ distribution of GAs and the expression of the key genes involved in peach flower formation in the apical meristem were studied during flowering differentiation. The results showed that induction of flowering in the Bayuecui peach occurred prior to 10 July in Beijing, China. Flower induction and further differentiation of the peach flower organs were significantly inhibited by leaf-spraying of GA 3 at a concentration of 100 mg L −1 during the induction stage. The flowering rate was only 11.67% after treatment. The distribution of GA 1 in the apical meristem varied during the process of flower bud differentiation. From 13 June to 25 July, the GA 1 signal from control plants was detected mainly in the vascular bundles at the base of the flower buds. No GA 1 signal was detected in the apical meristem. After treatment with GA 3, the distribution was similar to that of the control from 13 June to 3 July. On 13 July, a GA 1 signal was detected in the apical meristem accompanied by an increase in the GA 1 signal in the vascular bundles at the base of the flower buds. The GA 1 signal weakened significantly in both the vascular bundles and the apical meristem on 25 July. The expression of the genes PpLEAFY and MADS6 in flower buds could be detected only on 10 October in the GA 3-treated plants. The critical period for flower induction of Bayuecui peach in Beijing was in early July, during which time, leaf-spraying with 100 mg L −1 GA 3 could effectively inhibit flower induction and further differentiation of the flower buds. GA 1 in the gibberellin family was the suppressor for flower induction in peach. Its action was affected by the stage of flower bud differentiation. Expression of the key genes PpLEAFY and MADS6 involved in flower formation was inhibited by GA 3 treatment.