Abstract
(Effect of poultry carcas decontamination by acetic acid, citric acid and its combination to total plate count of Campylobacter sp).ABSTRACT. This research aimed to detect the total number of Campylobacter sp. on poultry carcass after decontamination by acetic acid, citric acid and combination of both. This research was conducted in the Laboratory of Veterinary Public Health Syiah Kuala University, Banda Aceh. The research was factorial completely randomized designed. Samples of poultry carcass were obtained from the Lamnyong market, Banda Aceh. Sixty poultry carcasses were divided into three groups of treatment and one group without treatment. Observation of each treatment was five time replicated at 0, 2, 4, 6 and 8 hours after immersion for ± 30 seconds in each of the decontamination material. Observation of Campylobacter sp. was done by inoculating every sample on CM0739 selective Campylobacter Blood free selective agar base and CCDA Selective supplement SR0155E. Measurements of Total Plate Count (TPC) Campylobacter sp according to Standard Plate Count (SPC). Data was variance analyzed (ANOVA) using SPSS. The results of research showed that the growing colonies of Campylobacter sp. were indicated by white colony with surrounded by black zone. Immersion of poultry carcass with acetic acid, citric acid and combination of the both and the observation time had no influence (P0,05) to ALT Campylobacter sp. The research was concluded that carcass to Campylobacter sp. The immersion by acetic acid, citric acid and combination of both and the observation time were not reduce Campylobacter sp. in poultry carcass.
Highlights
This research aimed to detect the total number of Campylobacter sp
citric acid and combination of both. This research was conducted in the Laboratory of Veterinary Public Health Syiah Kuala University
Samples of poultry carcass were obtained from the Lamnyong market
Summary
Materi Penelitian Sebanyak 60 karkas ayam pedaging yang diperoleh dari tempat pemotongan ayam di pasar Lamnyong Kota Banda Aceh digunakan sebagai materi penelitian. Efek Dekontaminasi Karkas Ayam Pedaging Menggunakan Asam Asetat, Asam Sitrat dan Kombinasinya...(Zalhendra Eka Putra, SST, M.Si., et al) selama 30 detik, selanjutnya karkas diambil bagian dada sebanyak 10 gram. Stern et al, (1992), setiap sampel daging bagian dada dihomogenkan dengan menggunakan stomacher ± 90 ml Buffered Pepton Water (BPW), selanjutnya sampel dimasukkan kedalam tabung reaksi berisikan ± 9 ml BPW. Sampel dilakukan pengeceran sampai 106 kemudian sampel dimasukkan ke dalam petri disc steril lalu diuji angka lempeng total dengan menggunakan Campylobacter sp. Blood free selective agar base yang sebelumnya dimasukkan CCDA Selective Supplement SR0155E 0,5 ml dalam 125 ml media. Yang tumbuh dalam cawan petri dihitung berdasarkan Standar Plate Count (Fardiaz, 1992). Perhitungan dilakukan pada koloni berwarna putih dengan dikelilingi zona kehitaman yang tumbuh pada media plate count agar Campylobacter sp. Perbedaan perlakuan dilanjutkan dengan uji BNt (Walpole, 1995)
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