Abstract
The production of alkaloids from Cereus peruvianus callus cultured in medium supplemented with different tyrosine concentrations, different 2,4-D/kinetin levels, and with NaCl was investigated using long-term callus culture. α- and β-Esterase isozyme patterns were also analyzed to investigate the differential gene expression in callus subcultured with the different tyrosine concentrations. The greatest amounts of alkaloids were obtained from callus maintained in medium containing 200 mg/L of tyrosine. Tyrosine induced a differential gene expression for the synthesis of specific α- and β -esterase isozymes. Two α-carboxylesterases (EC 3.1.1.1) and one α/β-arylesterase (EC 3.1.1.2) were induced in callus, one α/β-acetylesterase (EC 3.1.1.6) and two α-carboxylesterase were detected as more intensely stained while two α/β-carboxylesterases were absent or detected as more weakly stained bands after the addition of tyrosine as precursor
Highlights
Most investigations have used plant cell culture, with considerable advantage to living plants, for the production of secondary metabolites (Kutney, 1996; Sato et al, 2001)
We investigated the production of alkaloids from callus tissue cultured in medium supplemented with different tyrosine concentrations, at different 2,4-D/kinetin levels, and in medium supplemented with sodium chloride
Comparison of total alkaloids production from C. peruvianus callus tissues indicated that larger amounts of alkaloids were obtained from callus tissues maintained in medium containing 200 mg/L of tyrosine as supplement (Table 1)
Summary
Most investigations have used plant cell culture, with considerable advantage to living plants, for the production of secondary metabolites (Kutney, 1996; Sato et al, 2001). In the cactus Cereus peruvianus, comparison of total alkaloid production from plants with callus tissues indicated that larger amounts of alkaloids were obtained from long-term callus tissues.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
