Abstract
The present study reports a research with different concentrations and combinations of auxins and cytokinins to activate bud development and to obtain multiplication of Cattleya walkeriana (Orchidaceae) without an intermediate callus stage. Seedlings of C. walkeriana were cultured on B5 medium containing 2% sucrose and solidified with 0.65% agar, with factorial combinations of the auxins 1-naphthaleneacetic acid (NAA), 3-indolbutyric acid (IBA), and cytokinins 6-benzylaminopurine (BA) and N-(2-furanyl-methyl)-1-purine-6 amine (Kinetin) at concentrations of 0.0, 0.5, 1.0, and 1.5 mg/L. The highest number of buds with elongated leaves occurred by cultivating seedlings on medium containing different concentrations of IBA and BA, while the lowest number was observed in NAA and KIN combination. IBA alone yielded better results at the three tested concentrations, when compared to the total absence of growth regulators. The higher frequency of bud induction using BA and IBA combination indicated that this combinations were more effective for C. walkeriana in vitro multiplication using seedlings as explants
Highlights
Cattleya walkeriana Gardner (Orchidaceae) is a tropical epiphytic orchid native to the central region of Brazil
The present study reports the effects of different combinations of auxins and cytokinins for bud induction from C. walkeriana seedlings
The seedlings were placed in flasks (10 cm height x 4 cm diameter) containing 15 mL of B5 macro- and micro-salts, vitamins (Gamborg et al, 1968), and 2% sucrose, solidified with 0.65% agar, and the following combinations of the auxins 1naphthaleneacetic acid (NAA), 3-indolbutyric acid (IBA), and the cytokinins 6-benzylaminopurine (BA) and N-(2-furanyl-methyl)-1-purine-6 amine (Kinetin); four experiments (BA and IBA, BA and NAA, Kinetin and IBA, and Kinetin and NAA) were replicated three times at each factorial combination, i.e., 0.0, 0.5, 1.0, and 1.5 mg/L (BA 1.0 mg/L = 4.40 μM; Kinetin 1.0 mg/L = 4.60 μM; NAA 1.0 mg/L = 5.37 μM; IBA 1.0 mg/L = 4.92 μM)
Summary
Cattleya walkeriana Gardner (Orchidaceae) is a tropical epiphytic orchid native to the central region of Brazil. To date, there is no report on seedlings in vitro multiplication using growth regulators. The present study reports the effects of different combinations of auxins and cytokinins for bud induction from C. walkeriana seedlings.
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