Abstract
A reliable and rapid large scale micropropagation method has been established from the node, shoots tip and leaf explant of Chrysanthemum morifolium growing in field condition. Experiments were conducted to standardize the culture media with plant hormone for multiple shoot proliferation and rooting for obtaining plantlets with uniform characteristics like mother plant in terms of growth and habits. Different concentrations and combinations of auxins (IAA) and cytokinins (BAP, Kin) were used in MS for the above purpose. Maximum shoot regeneration was found in MS treated with 2.0 mg/l BAP both in node and shoot tip explants. In the above combination, nodal explants produced 16 initial shoots. Shoot tip explants produced 12 shoots and leaf segment produced 07 shoots. For in vitro rooting, different concentrations of IBA and NAA were used. Higher rooting percentage was recorded on MS fortified with 1.5 mg/l IBA. The rooted plantlets were hardened and successfully established in the soil. About 90% of the regenerated plantlets survived in the natural environment.
Highlights
Chrysanthemum morifolium commonly known as autumn queen belongs to the family Composaetae (Asteraceae) [1] is a perennial herb
In the present investigation attempts were made to standardize a reproducible protocol for in vitro clonal propagation of Chrysanthemum morifolium
For the development of multiple shoots three types of explants mentioned above was culture on to MS fortified with different concentration of cytokinins (BAP and Kn) as well as combination with a single concentration of IAA (0.5 mg/l)
Summary
Chrysanthemum morifolium commonly known as autumn queen belongs to the family Composaetae (Asteraceae) [1] is a perennial herb. Chrysanthemum is grown throughout the world both for cut flowers and as potted plant. There is a need to standardize a quicker method of propagation These problems have been solved by applying in vitro propagation techniques, which are routinely applied to the clonal propagation of a variety of horticultural plants including Chrysanthemum [6]. Corresponding author: Md Monirul Islam; E-mail: this technique is a solution for rapid propagation of selected plant species [9, 10 and 11]. Their having several reports regarding in vitro propagation of Chrysanthemum using various explants [12, 13 and 14]. The present study aims at developing a simple, rapid, and efficient high frequency regeneration techniques for potential application in large scale propagation of Chrysanthemum
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