Abstract
The enzyme activity and ultrastructure of osteoclasts in medullary bone cultured in the presence of parathyroid hormone (PTH) were examined using laying hens during the formative phase of the bone when an egg was in the magnum of the oviduct. Before and after the culturing in the absence of PTH, osteoclasts on the surface of medullary bone showed moderate activity for both acid phosphatase (ACP) and succinate dehydrogenase (SDH), and strong lactate dehydrogenase (LHD) activity. These cells lacked ruffled borders adjacent to the bone matrix. On the contrary, in the presence of PTH, ACP and SDH activity in osteoclasts increased after 3 hours of culture. Ruffled bordes also apperared at the peripheral cytoplasm apposed to the bone. After 12 hours of culture, the area of the ruffled borders further increased. After 24 hours of culture, almost all the osteoclasts had well developed ruffled borders. These results suggest that PTH stimulates osteoclastic resorption in the medullary bone of laying hens during the formative phase of the bone.
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