Abstract
ObjetivesThe aim of this study was to evaluate the effects of 3 different thawing procedures, as well as the effect of washing, on vitality, motility and sperm tail morphology from poor quality semen samples. Material and methodsThirteen poor quality semen samples were selected from different patients according to the parameters provided by the Word Health Organization. Samples were thawed according to the following protocols: a) 25 min at room temperature; b) 15 min at room temperature followed by 10 min at 37°C; c) 5 min at 4°C followed by 10 min at room temperature and 10 min at 37°C. In order to study the washing effects all thawed samples were evaluated before and after the washing procedure. Two pellets (150 μl pellet-1) were analyzed for each thawing protocol and vitality, motility and sperm tail morphological alterations were evaluated. ResultsThe results obtained showed that the thawing protocol did not show any effect (p >.05) on none of the evaluated characteristic. However, washing resulted in decreased (p <.001) vitality, active motility, increased morphological sperm tail alterations (p <.001). ConclusionsThese results suggest that washing rather than the thawing procedure could reduce sperm viability in poor quality sperm.
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