EDITING GENOMES VIA CRISPR/CAS9 AND APPLICATIONS IN MAIZE IMPROVEMENT

Abstract

The CRISPR/Cas9 system is a gene editing method capable of accurately locating and altering a specific sequence of a genome. Currently, this technique stands out as a promising biotechnological tool for the improvement of different crops, overcoming the limitations of conventional breeding methods and accelerating the development of plants’ agronomic characteristics. The basic principle of genome editing via CRISPR is to cause a double-stranded DNA break at a predetermined location by a sgRNA (single guide RNA) and through internal repair mechanisms cause mutations and, consequently, genetic variability. In prokaryotic organisms, it is an immune system that naturally protects against viral infection and has been modified to create different applications in higher organisms in the field of medicine and agriculture. In agriculture, it has been used in a large number of mono and dicotyledonous plants, including those of great commercial value such as soybean, cotton and maize. In maize, this technique has been used as a biotechnological tool for increasing production, quality, nutritional value, tolerance to biotic and abiotic stresses. CRISPR-Cas9 technology has enormous potential to contribute to increased food production with great benefit to the environment. This review explores the bases for the use of CRISPR/Cas9 technology to generate genetic variability and its use in the development of maize cultivars with better agronomic characteristics.