Abstract
large change in image brightness over a short spatial distance indicates the presence of an edge. The goal of edge detection of image is to mark the points in a digital image at which the luminous intensity changes sharply which refer changes in images properties .There are a number of techniques that might be used to edge detection of microscope image one of them is using AND binary operation.
 In this paper two techniques are used to edge detection of microscope images one of them is use Threshold with AND binary operation and the other is use Sobel operator with AND binary operation .The result show that the two techniques give a good result for edge detection of microscope image.
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