Abstract
FN (70, 80, 90 and 100 ug/ml) and the adherence was visualized by immunofluorescence using hyperimmune rabbit sera against T. solium activated oncospheres. In the other hand, only the CHO —K1 cells were preincubated with 80 ug/ml of FN for 20 h and then the T. solium oncospheres with and without FN were added on monolayer cell. FN fibrils and oncospheres were visualized by immunofluorescence using polyclonal anti-FN antibody produced in rabbit by UV light microscopy. Results: The adherence of T. solium oncospheres was increased from 70 ug/ml FN versus control. The fibrillar FN matrix on CHO—K1 cells were visualized over monolayer cell. Also, the adherence T. solium oncosphere with FN preincubate reacted with anti-FN antibody but with minor intensity versus control. Conclusion: These results suggest that Fn increases the adherence of oncosphere of T. solium serving as a bridge between the T. solium oncosphere and cell. Additonal characterization of T. solium oncosphere fibronectin binding protein that interact with host components, will further our understanding of the adherence and could be markers as candidate a vaccines.
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