ECOLOGICALLY BASED DISEASE MANAGEMENT TECHNIQUES IN BARLEY CULTIVATION IN THE CENTRAL BLACK SOIL REGION

Abstract

At present, there are microbiological Bacillus-based products used to preservevaluable micro biota and improving the level of biological soil productivity as wellas sustaining the local environment. The research focuses on discovering how someparticular biological products and autochthonous microorganisms influence theyield capacity of barley grown in the Black-Earth region of Russia. The researchobjectives included a search for autochthonous strains of microorganisms thatimprove resistance to diseases, estimation of how biological products contribute tothe quality of barley seeds, biological products effect on the spread and diseaseresistance and estimation of how biological products enhance the yield capacity ofbarley. The paper describes the results of identification of an autochthonousBacillus strains. PCR diagnostic methods were used to confirm the strain specificorigin of two sample cells extracted from soil (S1 and S2). The study involved theanalysis of micro biota of leached chernozem, which revealed the autochthonousstrain of Bacillus S1 having a germicidal effect. The S1 strain revealed Bacillussubtilis and Bacillus сеreus, while S2 revealed only Bacillus subtilis, as detected bythe method of molecular diagnostics based on using species-specific primers.Biological treatment of the seeds improved their sowing qualities, namely,germination readiness and germination capacity. In addition, it was found out thatsuch treatment improves the resistance to disease affection and spread. Bacillus S1,in particular, reduces the disease affection by 16,5 % and the disease spread 3,5 asmuch. Finally, the experiment demonstrated that biological treatment cancontribute to sustaining healthy environment for the plants and thus increase theiryield capacity.