Abstract
The degradation of heavy oil by two bacterial isolates from the sea water in oil-polluted Bisan Seto was investigated. Desulfurized “Class C” fule oil (the spilled fuel oil in “Mizushima Oil Spill”) before and after the degradation by coryneform Pl-1 and Moraxella Obl-1 was fractionated using silical gel columns. After 14 days at 25°C of incubation, the saturated fraction of the de-sulfurized fuel oil was more preferentially utilized than the aromatic fraction by both bacteria. When each fraction of the heavy oil was supplied as a sole carbon source, both bactria grew well on the saturated fraction but no growth was observed on the aromatic fraction. The results suggested that the saturated fraction of heavy oil was more suscetible to degradation than the aromatic fraction by these bacteria.
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