Abstract
The enzyme mimetic activity of nanomaterials has been applied in colorimetric assays and point-of-care diagnostics. Several nanomaterials have been exploited for their peroxidase mimetic activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. However, an efficient nanomaterial for the rapid and strong oxidation of TMB remains a strategic challenge. Therefore, in this study, we developed copper-loaded tin oxide (SnO2-Cu) nanocomposites that rapidly oxidize TMB. These nanocomposites have strong absorption at 650 nm and can be used for highly sensitive colorimetric detection. An environmentally friendly (green), rapid, easy, and cost-effective method was developed for the synthesis of these nanocomposites, which were characterized using ultraviolet-visible, energy-dispersive X-ray, and Fourier-transform infrared spectroscopy, as well as scanning electron microscopy. This is the first green synthesis of SnO2-Cu nanocomposites. Their enzyme mimetic activity, which was first studied here, was found to be strongly dependent on the temperature and pH value of the solution. The synthesized nanocomposites have the advantages of low cost, high stability, and ease of preparation for enzyme mimetic applications. Hence, SnO2-Cu nanocomposites are a promising alternative to peroxidase enzymes in colorimetric point-of-care diagnostics.
Highlights
Nanomaterials and nanocomposites have attracted the attention of scientists owing to their unique magnetic, chemical, optical, and electrical properties, which make them suitable for various applications, such their use as catalysts [1,2], photocatalysts, drugdelivery systems [3,4,5], colorimetric sensors [6], and antibacterials [7,8]
We focused on the development of an easy, cost-effective, and green method for the biosynthesis of SnO2 -Cu nanocomposites using a premature seed pod extract of Platycladus orientalis, and investigated the nanocomposites’ enzyme mimetic activity for the oxidation of TMB
The biosynthesized SnO2 -Cu nanocomposites were analyzed by field-emission scanning electron microscopy (FESEM) to determine their morphology and size
Summary
Nanomaterials and nanocomposites have attracted the attention of scientists owing to their unique magnetic, chemical, optical, and electrical properties, which make them suitable for various applications, such their use as catalysts [1,2], photocatalysts, drugdelivery systems [3,4,5], colorimetric sensors [6], and antibacterials [7,8]. Enzymes have inherent drawbacks such as high preparation and purification costs, low operational stability, sensitivity to environmental conditions, and difficulties in recycling To overcome these drawbacks, researchers are developing nanomaterials with peroxidase-like activities, functioning as artificial substitutes for enzymes with high stability. Researchers are developing nanomaterials with peroxidase-like activities, functioning as artificial substitutes for enzymes with high stability Various nanomaterials, such as gold nanoparticles [20], Pd nanoclusters [21], graphene oxide [22], and ZnO-Pd nanosheets have been developed [18]. We focused on the development of an easy, cost-effective, and green method for the biosynthesis of SnO2 -Cu nanocomposites using a premature seed pod extract of Platycladus orientalis, and investigated the nanocomposites’ enzyme mimetic activity for the oxidation of TMB. The synthesized SnO2 -Cu nanocomposites were characterized using various techniques and were found to have excellent peroxidase mimetic activity through their rapid oxidation of TMB with strong absorption at 650 nm
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have