Eco-Friendly Green Biosynthesis of Silver Nanoparticles (Or-AgNPs) Using Orange Peel (<i>Citrus sinensis</i>) Waste and Evaluation of their Antibacterial and Cytotoxic Activities

Abstract

Greener Nanotechnology research depends on the utilization of the reductive potency of a common byproduct of food and fruit processing industry which represent a cheap and reliable source of green reducing agents to be used in bio-nanosynthesis. One potential approach is based on silver bio-synthesis (AgNPs) using biological waste products. Recently, the production of bio-nanoparticles especially AgNPs has received enormous importance due to its good and potential physicochemical characteristics and the possibility of applications .The present work aimed to synthesis silver nanoparticles (AgNPs) using biological orange waste peels products (Citrus Sinensis) to evaluate its characterization, antimicrobial activity and cytotoxicity. The green synthesized silver nanoparticles were characterized by UV-visible spectroscopy and Scanning electron microscopy (SEM). Disk diffusion method was used for the study of antimicrobial activity of the bio-synthesized silver nanoparticles against the different bacterial and fungal strains. Characterization performed by Scanning Electron Microscope and UV visible spectrophotometer where showed the formation of spherical, and few agglomerated AgNPs forms as measured by UV–visible spectrophotometer in the range of 350-550 nm. Antimicrobial activities where showed positive activity against most of the tested human pathogenic bacteria and fungi with varying degrees and the cytotoxicity which performed against African Green Monkey Kidney cell lines (Vero) where CC50 for AgNO3 and AgNPs were ( 76.5±6.27 μl/100μl ) and (9.87±0.90μl/100μl) respectively.