Eco-friendly UV-spectrophotometric methods employing magnetic nano-composite polymer for the extraction and analysis of sexual boosters in adulterated food products: Application of computer-aided design.

Abstract

Solid phase extraction (SPE) techniques based on computationally designed magnetic-based multi-targeting molecular imprinted polymer (MT-MIP) and combined with UV spectrophotometric approaches provide advantages in the examination of counterfeit samples. The current work describes an innovative and sustainable methodology for the simultaneous determination of tadalafil (TAD) and dapoxetine hydrochloride (DAP) in counterfeited honey and instant coffee aphrodisiac products utilizing a SPE exploiting MT-MIP. Besides, an innovative UV spectrophotometric method capable of resolving TAD in its pharmaceutical binary mixtures with DAP was developed. A novel computational approach was implemented to tailor the synthesis and design of the MT-MIP particles. Herein; we applied a newly developed UV spectrophotometric method which was based on Fourier self-deconvolution (FSD) method coupled with isoabsorptive point for determination of TAD and DAP in pharmaceutical dosage form. Besides we applied a SPE process based on the MT-MIP designed particles, assisting in the analysis of both drugs in counterfeited food samples. The SPE process and the UV spectroscopic methodology were assessed regarding their greenness using the pioneering GAPI, AGREEprep and AGREE greenness assessing tools. The synthesized MT-MIP particles were characterized by scanning electron microscopy and energy-dispersive X-ray spectroscopy. The suggested spectrophotometric methods revealed a wider linear concentration range of 2-50 µg/mL with lower limits of detection in the range of 0.604 -0.994 µg/mL. Additionally, the suggested method demonstrated the utmost sensitivity and eco-friendliness for their target in its mixed dosage form and counterfeited food products. The SPE process and the developed analytical UV spectroscopic methodology were validated as per the ICH guidelines, and were found to be suitable for overseeing some counterfeiting activities in commercially available honey and instant coffee aphrodisiac products. An SPE based on MT-MIP magnetic-based polymer assisted with UV-spectroscopic method was successfully developed for analysis of tadalafil and dapoxetine hydrochloride in different matrices.