Eco Friendly Approach for Synthesis, Characterization and Biological Activities of Milk Protein Stabilized Silver Nanoparticles.

Sadanand Pandey,Corli De Klerk,Elvis Fosso-Kankeu,Misook Kang,Joonwoo Kim

doi:10.3390/polym12061418

Sadanand Pandey, Corli De Klerk + Show 3 more

Open Access

https://doi.org/10.3390/polym12061418

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Abstract

Today, the overall occurrence of re-emerging and rising illnesses has been a serious load on economies as well as public health. Here, we describe a simple, nontoxic and eco-friendly method for the synthesis of milk protein (MP)-stabilized silver nanoparticles (MP-s-AgNPs) using ultrahigh-temperature full cream milk. Highly stable AgNPs were prepared with a fair control over their size, without using any reducing or stabilizing agent, and their formation was attributed to the presence of the MP casein. Ag+ ion reduction was possibly caused by the MPs. The synthesized MP-s-AgNPs were characterized in detail by ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, transmission electron microscopy and dynamic light scattering. MP-s-AgNPs showed inhibitory activity against both Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative microorganisms (Salmonella typhi and Escherichia coli). Moreover, MP-s-AgNPs were found to be more toxic to bacteria than to fungi (Aspergillus fumigatus, Aspergillus ochraceus and Penicillium chrysogenum).

Highlights

Nanotechnology constitutes an impressive power tool in order to check out the overall darkest alternatives in reference to medical sciences in numerous ways such as imaging [1], catalysis [2], sensing [3,4,5], targeted drug delivery [6], gene delivery [7] and artificial implants [8]
The formation of milk protein (MP)-s-AgNPs was investigated by UV-Vis spectroscopy
As a result, when light interacts with such particles, the angular distribution of the scattered radiation is governed by the Lorenz–Mie theory

Summary

Introduction

Nanotechnology constitutes an impressive power tool in order to check out the overall darkest alternatives in reference to medical sciences in numerous ways such as imaging [1], catalysis [2], sensing [3,4,5], targeted drug delivery [6], gene delivery [7] and artificial implants [8]. AgNPs are used as deodorizers and disinfectants [9,10] by incorporating them into garments, bandages, coatings and food containers; in addition, they are used to purify drinking water, and recently, as an insecticide to control the attack of pests on various crops [11]. Most of these applications of AgNPs are attributed to their size- and shape-dependent unique chemical and physical characteristics [12,13,14]. AgNPs have been synthesized by borohydride reduction [19], acrylate/citrate reduction [20,21], polyol process [22,23], microwave irradiation [24,25], plant extract and vegetable oil-based process [26,27], photoreduction [28], Polymers 2020, 12, 1418; doi:10.3390/polym12061418 www.mdpi.com/journal/polymers

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