ECDYSTEROIDS AND FLAVONOIDS OF SILENE GRAEFFERI

Abstract

In this paper Silene graefferi Guss. is recommended for the first time as a promising source of flavonoids and is a well-known producer of ecdysteroids. This perennial species is adapted to the conditions of Western Siberia and has been successfully cultivated in the Siberian Botanical garden of TSU since 2015. It was interesting to study the composition and features of accumulation of biologically active substances during the growth and development of S. graefferi. It was shown that 20-hydroxyecdysone and polypodine B are present in the aerial and underground parts of introduced in culture S. graefferi plants during the entire vegetative development. An unidentified ecdysteroid was detected in the aerial part at the beginning of the vegetation and in the roots during the flowering phase. During vegetative development, common flavonoids and characteristic ones at different periods of plant development were found. The phase of maximum accumulation of ecdysteroids and flavonoids of S. graefferi is the beginning of vegetation, and the minimum is flowering. In the age dynamics the highest levels of biologically active substances in plants were noted at 2 and 3 years of life, then there is a general tendency to decrease with the age of the plant. From the aerial part of S. graefferi, 20-hydroxyecdysone, polypodine B and vitexin were isolated and identified by HPLC.