Abstract
Radish is a crucial vegetable crop of the Brassicaceae family with many varieties and large cultivated area in China. Radish is a cool season crop, and there are only a few heat tolerant radish varieties in practical production with little information concerning the related genes in response to heat stress. In this work, some physiological parameter changes of young leaves under short-term heat stress were detected. Furthermore, we acquired 1802 differentially expressed mRNAs (including encoding some heat shock proteins, heat shock factor and heat shock-related transcription factors), 169 differentially expressed lncRNAs and three differentially expressed circRNAs (novel_circ_0000265, novel_circ_0000325 and novel_circ_0000315) through strand-specific RNA sequencing technology. We also found 10 differentially expressed miRNAs (ath-miR159b-3p, athmiR159c, ath-miR398a-3p, athmiR398b-3p, ath-miR165a-5p, ath-miR169g-3p, novel_86, novel_107, novel_21 and ath-miR171b-3p) by small RNA sequencing technology. Through function prediction and enrichment analysis, our results suggested that the significantly possible pathways/complexes related to heat stress in radish leaves were circadian rhythm-plant, photosynthesis—antenna proteins, photosynthesis, carbon fixation in photosynthetic organisms, arginine and proline metabolism, oxidative phosphorylation, peroxisome and plant hormone signal transduction. Besides, we identified one lncRNA–miRNA–mRNAs combination responsive to heat stress. These results will be helpful for further illustration of molecular regulation networks of how radish responds to heat stress.
Highlights
Due to the significant increase in greenhouse gas emissions from human activities, especially the oxides of carbon dioxide, methane, chlorofluorocarbons and nitrogen, the global warming and the rising temperature have made plants in danger of high-temperature stress [1]
In order to further excavate the DE mRNA related to HS in the cultivar “Huoche”, we found that the heat stress transcription factor A-8-like (LOC108834589), heat shock factor-binding protein 1-like (LOC108833879), hsp70 nucleotide exchange factor fes1 (LOC108859078), hsp70 nucleotide exchange factor fes1-like (LOC108857317), 28 kDa heat- and acid-stable phosphoprotein (LOC108806935) and HEAT repeat-containing protein 5B%2C (LOC108817282) were differentially expressed (Table 1)
We detected the changes of superoxide dismutase (SOD), POD and MDA under heat stress
Summary
Due to the significant increase in greenhouse gas emissions from human activities, especially the oxides of carbon dioxide, methane, chlorofluorocarbons and nitrogen, the global warming and the rising temperature have made plants in danger of high-temperature stress [1]. Plant osmotic adjustment substances mainly include amino acids, soluble sugars, soluble proteins and soluble phenols [10] These studies suggest that the changes in soluble sugar, chlorophyll, free proline and MDA contents, as well as SOD and POD activities under HS, can be used as indicators for the evaluation and screening of crop heat tolerance. More and more studies have shown that non-coding RNAs (ncRNAs; including lncRNAs, miRNAs and circRNAs) play important roles in organisms [18], and the expression of some genes responsive to stress is regulated by ncRNAs [19,20]. We obtained the data of soluble sugar, chlorophyll, free proline and MDA contents as well as SOD and POD activities, and transcriptome including mRNA, non-coding RNA from radish leaves under high temperature stress. The results would provide valuable clues for deep molecular analysis of radish heat response
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
