Abstract
ObjectiveThe objective of this study was to define how acute anterior cruciate ligament (ACL) complete injury remnant would potentially alter specific mRNA expression of angiogenesis and collagen synthesis factor by early protective joint motion.MethodsThirty‐six Wistar rats were divided into 3 groups: Control, Sham operated, and early protective joint motion. In the joint motion group, ACL was surgically transected and then tied up the joint with nylon string to put brake on forward slides. Each group of 4 rats was sacrificed at 1 day, 3 days, and 5 days postoperatively. Total RNA from the ACL remnant was reverse transcribed into cDNA. Real‐time RT‐PCR was performed using rat specific primer sets for COL 1, COL 3, VEGF and housekeeping gene (B‐actin). Differences in the Ct values between the tested mRNA and the B‐actin mRNA were calculated to determine the relative expression revels.ResultIn the early protective joint motion group, mRNA levels for COL 1 and COL 3 increased all time‐points (1 day, 3 days, and 5 days) and 2 time‐points (1 day, and 3 days), respectively. No significant differences in VEGF mRNA expression were found in this protocol.DiscussionIn the present study, we demonstrated acute ACL complete rupture remnant has healing potential. Furthermore controlling malfunction and inducing normal joint movement in acute stage make it possible.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call