Early molecular events of Autosomal Dominant Alzheimer’s Disease in marmosets with PSEN1 mutations

Abstract

Listen

Translate article

AbstractBackgroundFundamental questions remain about the key mechanisms that initiate Alzheimer’s disease (AD) and the factors that promote its progression. Here we report the successful generation of the first genetically‐engineered marmosets that carry knock‐in (KI) point mutations in the presenilin‐1 (PSEN1) gene that can be studied from birth throughout lifespan.MethodCRISPR/Cas9 was used to generate marmosets with C410Y or A426P point mutations in PSEN1. Founders and their germline offspring are comprehensively studied longitudinally using non‐invasive measures including behavior, biomarkers, neuroimaging, and multi‐omics signatures.ResultPrior to adulthood, increases in plasma Aβ were observed in PSEN1 mutation carriers relative to non‐carriers. Analysis of brain revealed alterations in several enzyme‐substrate interactions within the gamma secretase complex prior to adulthood.ConclusionMarmosets carrying KI point mutations in PSEN1 provide the opportunity to study the earliest primate‐specific mechanisms that contribute to the molecular and cellular root causes of AD onset and progression.