Early detection of the ovarian cancer with a label-free and disposable ITO-PET based immunosensor platform

Abstract

Alpha fetoprotein (AFP), a popular biomarker for ovarian cancer, was the target of this studyto create an immunosensor based on electrochemical impedance spectroscopy (EIS). For this purpose, a disposable Indium tin oxide coated PET (In2O3/SnO2, ITO-PET) electrode was used as a basic electrode for working of the triple electrode system and the electrodes were modified with 3-(Trimethoxy silyl) propyl methacrylate (3- TMSPM) silanization agent. Although there are studies on the determination of AFP, the determination of AFP with a cheap, flexible and sensitive electrode system such as ITO-PET in this study makes the study remarkable. With the designed biosensors, AFP protein determination at a wide concentration range (0.05 pg/mL-100 pg/mL) could be successfully performed. To prove that the designed immunosensor was constructed correctly, linear range, repeatability, reproducibility and regeneration capacity (characterization studies) were investigated. Obtaining successful results in the storage capacity studies of the biosensor, which has high reproducibility and repeatability, showed that it also has potential for commercial use. The high specificity of the biosensor also proves its reliability. Finally, the performance of the biosensor in commercial human serum samples was also evaluated.