Abstract
Rooted spruce cuttings from three clones were sprayed daily with sulfuric acid at pH 2.5 for one month or treated with automobile exhaust for one hour. Foliage samples were extracted and analyzed by high performance liquid chromatography. Chromatographic peaks representing unidentified leaf constituents from treated and control trees were compared. Discriminant analysis indicated a weak but significant difference and Canonical discriminant analysis indicated clear differences between treatments and control. Similar differences were found between the three clones. Visible symptoms of damage were not evident even four months after treatment. This result is important because it demonstrates the possibility of using unidentified plant constituents to detect damage to plants even before visible symptoms appear.
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