Early Conception Factor (ECF) Dipstick Test™ for Non-conception Determination in Cattle

Abstract

The Early Conception Factor (ECF) Test is a lateral-flow technology using a nitrocellulose matrix that allows for the measurement of glycoprotein present in serum or milk from pregnant cows between 6 and 15 days after breeding. This is a patented process utilizing monoclonal antibodies to the early conception factor as well as polyclonal antibodies conjugated to gold colloids. The test is performed by placing 1 drop of either serum or milk using a supplied dropper pipette into the sample well, followed by addition of 4 drops of a running buffer prepared to enhance the specificity and sensitivity of the test. After a 2-hour incubation at room temperature, the presence of 1 line indicates an open cow while the presence of 2 lines indicates the cow has conceived. Cows numbered 436, used for the serum testing portion of this project and 275 cows were used for the milk portion.