Abstract
The effects of apoptosis induction on glucose and phospholipid metabolite levels in cancer were studied using human colon adenocarcinoma cells (HT-29). Apoptosis was induced by co-incubation with 200 U/ml tumor necrosis factor (TNF)-α for 4, 8 or 15 h, after sensitization with 500 U/ml interferon (IFN)-γ for 7 h. Perchloric acid extracts were analyzed by 1H and 31P nuclear magnetic resonance (NMR) spectroscopy. Significantly increased lactate and NTP (all nucleoside 5’-triphosphates) signals were detected 4 h after apoptosis-inducing IFN-γ/TNF-α treatment, but not in cells which were TNF-α-treated without IFN-γ preincubation. Simultaneous lactate and NTP changes, if confirmed in vivo, may serve as early, non-invasive markers of treatment response in some tumors.
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