Abstract
An important requirement for the analysis of NMR spectra is the availability of a reference resonance so that reliable chemical shifts can be obtained. Of particular importance for in vivo 31P nuclear magnetic resonance spectroscopy is the determination of the inorganic phosphate chemical shift because the intracellular pH of the tissue ca then be estimated. This chapter discusses a study to elaborate the estimation of cerebral intracellular pH by 31P and 1H nuclear magnetic resonance spectroscopy. The muscle data were collected from the posterior calves of seven male subjects aged from 5 to 9 years. Neonatal brain spectra were obtained in the course of routine investigations of perinatal metabolism. Spectra were acquired using a double tuned 7 cm planar surface coil placed immediately superior to the pinna. Data were accumulated in batches of 256 FIDs and the final number of batches added together to produce the spectrum was decided by clinical criteria. 31P spectra were bracketed in spectra which were used as an indication of the stability of the examination conditions. The muscle data were processed using moderate profile correction and 12 Hz exponential line broadening to enhance the SNR. Processing of the neonatal brain spectra employed slightly more profile correction due to the presence of broad spectral components from relatively immobile 31P species.
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