DYSLIPIDEMIA, POLYMORPHIC GENETIC MARKERS AND CARDIOVASCULAR RISK IN PATIENTS WITH TYPE 2 DIABETES: PP.17.140

Abstract

Objective: Diabetic dyslipidemia confers risk for the development of cardiovascular complications in patients with type 2 diabetes mellitus. The aim of study was to determine an association between polymorphic variants of apolipoprotein (apo) gene cluster on chromosome 11q23, atherogenic lipid disorders and cardiovascular diseases in type 2 diabetic patients. Materials and Methods: We examined 275 patients with type 2 diabetes (78 males and 197 females, mean age 57 ± 0,4 years). Serum lipids were detected by enzymatic method. The genotypes of apoA1 (G-75A, C83T), apoC3 (SstI) and apoA5 (S19W, T-1131C) were identified by polymerase chain reaction - restriction fragment length polymorphism method. Results: Atherogenic dyslipidemia was revealed in 51,1% diabetic patients. Most of the patients had arterial hypertension (88,4%), 46,5% -coronary heart disease (CHD). The rare allele frequency of apoA5 S19W variant was significantly higher in patients with hypertriglyceridemia (HTG) (p = 0,004) and in patients with HTG and low HDL-cholesterol levels (p = 0,007) than in normolipidemic patients (controls). The apoA1 -75A allele frequency was significantly higher in female patients with HTG and low HDL-cholesterol levels than in controls (p = 0,028). The genotype and allele frequencies of apoA1, apoC3 and apoA5 polymorphic variants did not differ between patients with hypertension, CHD and patients without cardiovascular diseases. Conclusion: Our findings reveal an association of apo-genes polymorphic variants with atherogenic lipid profile but not with cardiovascular diseases in patients with type 2 diabetes mellitus.